Histone H4 anticorps (acLys12)

N° du produit ABIN5706795

N° du produit (Fournisseur): 600-401-p06

Cet anticorps anti-Histone H4 est un anticorps Lapin Polyclonal détectant Histone H4 dans WB, IHC, IF, DB, FM et MA. Adapté pour Humain.

Aperçu rapide pour Histone H4 anticorps (acLys12) (ABIN5706795)

Antigène: Histone H4

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Dot Blot (DB), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit anti-Histone H4 anticorps

Épitope: acLys12

N° du produit (Fournisseur): 600-401-p06

Fournisseur: Rockland

Fonction: Histone H4 K12ac Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Alfalfa based on 100 % sequence homology.

Attributs du produit: Synonyms: rabbit anti-Histone H4 Ac Lys12 antibody, H4K12ac, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

Purification: Anti-Histone H4 [ac Lys12] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H4 [ac Lys12] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 12 of human Histone H4.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-Histone H4 K12 ac antibody is tested for Western Blot, Dot Blot, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~11 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1.0 μg/mL

IF Microscopy Dilution: 1:10

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.69 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Histone H4

Antigène: Histone H4

Sujet: Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys5 (H4 Lys5Ac) has been linked to transcriptional activation and DNA repair. Newly assembled histones are typically acetylated on H4 at lysine 5 and 12. The enzyme histone acetyltransferase 1 (HAT1) is the primary modulator, and these marks are necessary for complete chromatin assembly. Research suggests that [ac Lys12] is associated with memory repair and telomere replication. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

ID gène: 121504

NCBI Accession: NP_001029249

UniProt: P62805